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PCR Primers for Amplification of ORFs and re-PCRs

Sequence of Plasmid/ORF Junctions

5' C TAT CTA TTC GAT GAT GAA GAT ACC CCA CCA AAC CCA AAA AAA GAG ATC GAA TTC CAG CTG
ACC ACC
ATG XXX XXX XXX XXX XXX XXX *** CAT GGC AAT TCC CGG GGA TCC GTC GAC CTG CAG
AGA TCT ATG AAT CGT AGA TAC TGA AAA ACC CCG CAA G 3'


Where
ATG represents the actual start codon of the yeast ORF.
XXX's represent the ORF-specific sequences.
***'s represent one of the 3 stop codons.

Primers and Re-PCR Primers Used for Amplifying Yeast ORFs and Their "Common Tails"

Forward primer
5' AATTCCAGCTGACCACCATG 3'

Reverse primer
5' GATCCCCGGGAATTGCCATG 3'


Re-PCR Forward primer
5' CTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAGATCGAATTCCAGCTGACCACCATG 3'

Re-PCR Reverse primer
5' CTTGCGGGGTTTTTCAGTATCTACGATTCATAGATCTCTGCAGGTCGACGGATCCCCGGGAATTGCCATG 3'
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  • Research
    • DNA Technology
    • RNA Technology
    • Protein Technology
    • Phage Technology
    • Synthetic Biology
    • Previous Projects
    • Yeast Resource Center
  • Members
  • Publications
  • Resources
    • Cloning 6000 ORFs
    • PCR Primers
    • Recombination Cloning Protocols
    • Vector pOBD2
    • Vector pOAD
    • Strains Used in Two-Hybrid Screens
  • Contact
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