2021
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Andrews, B. and Fields, S. (2021) Balance between promiscuity and specificity in phage λ host range. The ISME Journal, in press.
Download PDF Jores, T., Tonnies, J., Wrightsman, T., Buckler, E.S., Cuperus, J.T., Fields, S. and Queitsch, C. (submitted) Synthetic promoter designs enabled by a comprehensive analysis of plant core promoters. Dorrity, M.W., Alexandre, C., Hamm, M., Vigil, A.-L., Fields, S., Queitsch, C. and Cuperus, J. (submitted) The regulatory landscape of Arabidopsis thaliana roots at single-cell resolution. Morton, E.A., Dorrity, M.W., Zhou, W., Bubb, K., Fields, S. and Queitsch, C. (submitted) Transcriptional re-wiring by mutation of the yeast Hsf1 oligomerization domain. Zhou, W., Melamed, D., Wickens, M. and Fields, S. (submitted) Expanding the binding specificity for RNA recognition by a PUF domain. Hills, E., Brandsen, B.M. and Fields, S. (submitted) A high-resolution sequence–function map of the lasso peptide klebsidin. |
2020
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Zhou, W., Dorrity, M.W., Bubb, K.L., Queitsch, C. and Fields, S. (2020) Binding and regulation of transcription by yeast Ste12 variants to drive mating and invasion phenotypes. Genetics 214:397-407.
Download PDF Dorrity, M.W., Saunders, L.M., Queitsch, C., Fields, S. and Trapnell, C. (2020) Dimensionality reduction by UMAP to visualize physical and genetic interactions. Nature Communications 11:1537. Download PDF Andrews, B. and Fields, S. (2020) Distinct patterns of mutational sensitivity for λ resistance and maltodextrin transport in Escherichia coli LamB. Microbial Genomics 6:e000364. Download PDF Jores, T., Tonnies, J., Dorrity, M.W., Cuperus, J., Fields, S. and Queitsch, C. (2020) Identification of plant enhancers and their constituent elements by STARR-seq in tobacco leaves. Plant Cell 32:2120-2131. Download PDF |
2019 |
Dorrity, M.W., Queitsch, C. and Fields, S. (2019) High-throughput identification of dominant negative polypeptides in yeast. Nature Methods 16: 413-416.
Download PDF Jean-Baptiste, K., McFaline-Figueroa, J.L., Alexandre, C.M., Dorrity, M.W., Saunders, L., Bubb, K.L., Trapnell, C., Fields, S., Queitsch, C. and Cuperus, J.T. (2019) Dynamics of gene expression in single root cells of A. thaliana. Plant Cell 31: 993-1011. Download PDF |
2018 |
Alexandre, C.M., Urton, J.R., Jean-Baptiste, K., Huddleston, J.L., Dorrity, M.W., Cuperus, J.C., Sullivan, A.M., Bemm, F., Jolic, D., Arsovski, A.A., Thompson, A., Nemhauser, J.L., Fields, S., Weigel, D., Bubb, K.L. and Queitsch, C. (2018). Complex relationships between chromatin accessibility, sequence divergence, and gene expression in A. thaliana. Molecular Biology and Evolution 35: 837-854.
Download PDF Payea, M.J., Sloma, M.F., Kon, Y., Young, D.L., Guy, M.P., Zhang, X., De Zoysa, T., Fields, S., Mathews, D.H. and Phizicky, E.M. (2018) Widespread temperature sensitivity and tRNA decay due to mutations in a yeast tRNA. RNA 24: 410-422. Download PDF Dorrity, M.W., Cuperus, J.T., Carlisle, J.A., Fields, S. and Queitsch, C. (2018) Preferences in a trait decision determined by transcription factor variants. Proceedings of the National Academy of Sciences U.S.A. 115: E7997-E8006. Download PDF Starita, L.M., Islam, M.M., Banerjee, T., Adamovich, A.I., Gullinsrud, J., Fields, S., Shendure, J. and Parvin, J.D. (2018) A multiplexed homology-directed DNA repair assay reveals the impact of more than 1,000 BRCA1 missense substitution variants on protein function. American Journal of Human Genetics 103: 498-508. Download PDF Brandsen, B.M., Mattheisen, J., Noel, T. and Fields, S. (2018) A biosensor strategy for E. coli based on ligand-dependent stabilization. ACS Synthetic Biology 7; 1990-1999. Download PDF Zimmerman, S.M., Kon, Y., Hauke, A.C., Ruiz, B.Y., Fields, S. and Phizicky, E.M. (2018) Conditional accumulation of toxic tRNAs to cause amino acid misincorporation. Nucleic Acids Research, 24: 410-422. Download PDF Jester, B.W., Tinberg, C.E., Rich, M.S., Baker, D. and Fields, S. (2018) Engineered biosensors from dimeric ligand-binding domains. ACS Synthetic Biology 7: 2457-2467. Download PDF |
2017 |
Cuperus JT, Groves B, Kuchina A, Rosenberg AB, Jojic N, Fields S, Seelig G. Deep learning of the regulatory grammar of yeast 5' untranslated regions from 500,000 random sequences. Genome Res. 2017 Dec;27(12):2015-2024. doi: 10.1101/gr.224964.117. Epub 2017 Nov 2. PubMed PMID: 29097404; PubMed Central PMCID: PMC5741052.
Download PDF Bhagavatula G, Rich MS, Young DL, Marin M, Fields S. A Massively Parallel Fluorescence Assay to Characterize the Effects of Synonymous Mutations on TP53 Expression. Mol Cancer Res. 2017 Oct;15(10):1301-1307. doi: 10.1158/1541-7786.MCR-17-0245. Epub 2017 Jun 26. PubMed PMID: 28652265; PubMed Central PMCID: PMC5626615. Download PDF |
2016 |
Gamble CE, Brule CE, Dean KM, Fields S, Grayhack EJ. Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast. Cell. 2016;166(3):679-90.
Download PDF Rich MS, Payen C, Rubin AF, Ong GT, Sanchez MR, Yachie N, et al. Comprehensive Analysis of the SUL1 Promoter of Saccharomyces cerevisiae. Genetics. 2016;203(1):191-202. Download PDF Shendure J, Fields S. Massively Parallel Genetics. 2016 Jun;203(2):617-9. Download PDF Taylor ND, Garruss AS, Moretti R, Chan S, Arbing MA, Cascio D, Rogers JK, Isaacs FJ, Kosuri S, Baker D, Fields S, Church GM, Raman S. Engineering an allosteric transcription factor to respond to new ligands. Nature Methods. 2016 Feb. 13(2): 177-183 Download PDF |
2015 |
Feng J, Jester BW, Tinberg CE, Mandell DJ, Antunes MS, Chari R, Morey KJ, Rios X, Medford JI, Church GM, Fields S, Baker D. A general strategy to construct small molecule biosensors in eukaryotes. Elife. 2015 Dec 29;4. pii: e10606. doi: 10.7554/eLife.10606.
Download PDF Lee, C., Banerjee, T., Gillespie, J., Ceravolo, A., Parvinsmith, M.R., Starita, L.M., Fields, S., Toland, A.E. and Parvin, J.D. Functional analysis of BARD1 missense variants in homology-directed repair of DNA double strand breaks. Human Mutation. 2015 Dec; 36(12):1205-14. Young DL, Fields S. The role of functional data in interpreting the effects of genetic variation. Mol Biol Cell. 2015 Nov 5;26(22):3904-8. Download PDF Starita LM, Young DL, Islam M, Kitzman JO, Gullingsrud J, Hause RJ, Fowler DM, Parvin JD, Shendure J, Fields S. Massively Parallel Functional Analysis of BRCA1 RING Domain Variants. Genetics. 2015 Mar 30. Download PDF Cuperus JT, Lo RS, Shumaker L, Proctor J, Fields S. A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae. ACS Synth Biol. 2015 Mar 17. Download PDF Melamed D, Young DL, Miller CR, Fields S. Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites. PLoS Genet. 2015 Feb 11;11(2):e1004918. Download PDF Kitzman JO, Starita LM, Lo RS, Fields S, Shendure J. Massively parallel single-amino-acid mutagenesis. Nat Methods. 2015 Mar;12(3):203-6. Download PDF |
2014 |
Vidal M, Fields S. The yeast two-hybrid assay: still finding connections after 25 years. Nat Methods. 2014 Dec;11(12):1203-6.
Download PDF Raman S, Taylor N, Genuth N, Fields S, Church GM. Engineering allostery. Trends Genet. 2014 Dec;30(12):521-8. Download PDF Fowler DM, Stephany JJ, Fields S. Measuring the activity of protein variants on a large scale using deep mutational scanning. Nature Protocols. 2014 Sep;9(9):2267-84. Fowler DM, Fields S. Deep mutational scanning: a new style of protein science. Nature Methods. 2014 Aug;11(8):801-7. Guy, M.P., Young, D.L., Payea, M.J., Zhang, X., Kon, Y., Dean, K.M., Grayhack, E.J., Mathews, D.H., Fields, S. and Phizicky, E.M. Identification of the determinants of tRNA function and susceptibility to rapid tRNA decay by high-throughput in vivo analysis. Genes Dev. 2014 Aug 1;28(15):1721-32. Garrey, S.M., Katolik, A., Prekeris, M., Li, X., York, K., Bernards, S., Fields, S., Zhao, R., Damha, M.J. and Hesselberth, J.R. A homolog of lariat-debranching enzyme modulates turnover of branched RNA. RNA. 2014 Aug;20(8):1337-48 Fields, S. Would Fred Sanger get funded today? Genetics. 2014 Jun 1;197(2):435-439. |
2013 |
Bennett CL, Chen Y, Vignali M, Lo RS, Mason AG, Unal A, Huq Saifee NP, Fields S, La Spada AR. Protein interaction analysis of senataxin and the ALS4 L389S mutant yields insights into senataxin post-translational modification and uncovers mutant-specific binding with a brain cytoplasmic RNA-encoded peptide. PLoS One. 2013 Nov 11;8(11).
Download PDF Melamed D, Young DL, Gamble CE, Miller CR, Fields S. Deep mutational scanning of an RRM domain of the Saccharomyces cerevisiae poly(A)-binding protein. RNA. 2013 Nov;19(11):1537-51. Epub 2013 Sep 24. Download PDF Kim I, Miller CR, Young DL, Fields S. High-throughput Analysis of in vivo Protein Stability. Mol Cell Proteomics. 2013 Jul 29. [Epub ahead of print]. Download PDF Skelly DA, Merrihew GE, Riffle M, Connelly CF, Kerr EO, Johansson M, Jaschob D, Graczyk B, Shulman NJ, Wakefield J, Cooper SJ, Fields S, Noble WS, Muller EG, Davis TN, Dunham MJ, Maccoss MJ, Akey JM. Integrative phenomics reveals insight into the structure of phenotypic diversity in budding yeast. Genome Res. 2013 Sep;23(9):1496-504. doi: 10.1101/gr.155762.113. Epub 2013 May 29. Download PDF Gold MG, Fowler DM, Means CK, Pawson CT, Stephany JJ, Langeberg LK, Fields S, Scott JD. Engineering A-kinase anchoring protein (AKAP)-selective regulatory subunits of protein kinase A (PKA) through structure-based phage selection. J Biol Chem. 2013 Jun 14;288(24):17111-21. Download PDF Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Nat Methods. 2013 Jul;10(7):676-82. doi: 10.1038/nmeth.2519. Epub 2013 Jun 9. Download PDF Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB, Shendure J, Brzovic PS, Fields S, Klevit RE. Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis. Proc Natl Acad Sci U S A. 2013 Mar 18. Download PDF |
2012 |
Araya CL, Fowler DM, Chen W, Muniez I, Kelly JW, Fields S. A fundamental protein property, thermodynamic stability, revealed solely from large-scale measurements of protein function. Proc Natl Acad Sci. 2012 Oct 16;109(42):16858-63.
Download PDF Duan Z, Andronescu M, Schutz K, Lee C, Shendure J, Fields S, Noble WS, Anthony Blau C. A genome-wide 3C-method for characterizing the three-dimensional architectures of genomes. Methods. 2012 Jul 6. Download PDF McGarvey KM, Queitsch K, and Fields S. Wide variation in antibiotic resistance proteins identified by functional metagenomic screening of a soil DNA library. Applied and Environmental Microbiology. 2012 Mar;78(6):1708-14. Epub 2012 Jan 13. Download PDF McKinlay A, Gerard W, Fields S. Global analysis of RNA oxidation in Saccharomyces cerevisiae. Biotechniques. 2012 Feb;52(2):109-11. Download PDF Starita, L.M., Lo, R.S., Eng, J.K., Von Haller, P.D. and Fields, S. Sites of ubiquitin attachment in Saccharomyces cerevisiae. Proteomics. 2012 Jan;12(2):236-240. Epub 2011 Nov 22. Download PDF |
2011 |
McKinlay, A., Araya, C.L. and Fields, S. Genome-wide analysis of nascent transcription in Saccharomyces cerevisiae. G3: Genes, Genomes, Genetics. 2011 Dec;1(7):549-58. Epub 2011 Dec 1.
Download PDF Lo, M., Brett, C.L., Plemel, R., Vignali, M., Fields, S., Gonen, T., and Merz, A.J. Intrinsic tethering activity of endosomal Rab proteins. Nature Structural and Molecular Biology. 2011 Dec 11;19(1):40-7. Download PDF Fowler DM, Araya CL, Gerard W, Fields S. Enrich: Software for Analysis of Protein Function by Enrichment and Depletion of Variants. Bioinformatics. Bioinformatics. 2011 Dec 15;27(24):3430-1. Epub 2011 Oct 17. Download PDF Araya CL, Fowler DM. Deep mutational scanning: assessing protein function on a massive scale. Trends in Biotechnology. 2011 Sep;29(9):435-42. Epub 2011 May 10. Download PDF Brown HF, Wang L, Khadka S, Fields S, LaCount DJ. A densely overlapping gene fragmentation approach improves yeast two-hybrid screens for Plasmodium falciparum proteins. Mol Biochem Parasitol. 2011 Jul-Aug;178(1-2):56-9. Epub 2011 Apr 20. Download PDF Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, Ohi MD, Gould KL. Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS One. 2011 Feb 28;6(2):e16719. Download PDF Fowler DM, Cooper SJ, Stephany JJ, Hendon N, Nelson S and Fields S. Suppression of statin effectiveness by copper and zinc in yeast and human cells. Mol. BioSyst. 2011, Feb;7(2):533-544. Download PDF Piña FJ, O'Donnell AF, Pagant S, Piao HL, Miller JP, Fields S, Miller EA, Cyert MS. Hph1 and Hph2 are novel components of the Sec63/Sec62 posttranslational translocation complex that aid in vacuolar proton ATPase biogenesis. Eukaryot Cell. 2011 Jan;10(1):63-71. Download PDF |
2010 |
Yasutis, K., Vignali, M., Ryder, M., Tameire, F., Dighe, S.A., Fields, S. and Kozminski, K.G. Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain. Mol Biol Cell. 2010 Dec 15;21(24): 4373–4386.
Fields, S. and Johnston, M. Genetic Twists of Fate. Cambridge, MA: The MIT Press. 2010. Fowler DM, Araya CL, Fleishman SJ, Kellogg EH, Stephany JJ, Baker D, Fields S. High-resolution mapping of protein sequence-function relationships. Nat Methods. 2010 Sep;7(9):741-6. Download PDF Cooper SJ, Finney GL, Brown SL, Nelson SK, Hesselberth J, Maccoss MJ, Fields S. High-throughput profiling of amino acids in strains of the Saccharomyces cerevisiae deletion collection. Genome Res. 2010 Sep;20(9):1288-96. Download PDF Duan Z, Andronescu M, Schutz K, McIlwain S, Kim YJ, Lee C, Shendure J, Fields S, Blau CA, Noble WS. A three-dimensional model of the yeast genome. Nature. 2010 May 20;465(7296):363-7. Download PDF Araya CL, Payen C, Dunham MJ, Fields S. Whole-genome sequencing of a laboratory-evolved yeast strain. BMC Genomics. 2010 Feb 3;11:88. Download PDF Schutz K, Hesselberth JR, Fields S. Capture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. RNA. 2010 Mar;16(3):621-31. Download PDF Supplemental Figures Supplemental Figure Legends Supplemental Raw Sequence Data |
2009 |
Fields S. Interactive learning: lessons from two hybrids over two decades. Proteomics. 2009 Dec;9(23):5209-13.
Download PDF Hesselberth JR, Chen X, Zhang Z, Sabo PJ, Sandstrom R, Reynolds AP, Thurman RE, Neph S, Kuehn MS, Noble WS, Fields S, Stamatoyannopoulos JA. Global mapping of protein-DNA interactions in vivo by digital genomic footprinting. Nat Methods. 2009 Apr;6(4):283-9. Download PDF Lacount DJ, Schoenfeld LW, Fields S. Selection of yeast strains with enhanced expression of Plasmodium falciparum proteins. Mol Biochem Parasitol. 2009 Feb;163(2):119-22. Download PDF |
2008 |
Vignali M, McKinlay A, LaCount DJ, Chettier R, Bell R, Sahasrabudhe S, Hughes RE, Fields S. Interaction of an atypical Plasmodium falciparum ETRAMP with human apolipoproteins. Malar J. 2008 Oct 20;7:211.
Download PDF Supplemental Data Brett CL, Plemel RL, Lobinger BT, Vignali M, Fields S, Merz AJ. Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase. J Cell Biol. 2008 Sep 22;182(6):1141-51. Download PDF Supplemental Data Khoury CM, Yang Z, Li XY, Vignali M, Fields S, Greenwood MT. A TSC22-like motif defines a novel antiapoptotic protein family. FEMS Yeast Res. 2008 Jun;8(4):540-63. Download PDF Steffen KK, MacKay VL, Kerr EO, Tsuchiya M, Hu D, Fox LA, Dang N, Johnston ED, Oakes JA, Tchao BN, Pak DN, Fields S, Kennedy BK, Kaeberlein M. Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4. Cell. 2008 Apr 18;133(2):292-302. Download PDF Supplemental Data |
2007 |
Wong J, Nakajima Y, Westermann S, Shang C, Kang JS, Goodner C, Houshmand P, Fields S, Chan CS, Drubin D, Barnes G, Hazbun T. A protein interaction map of the mitotic spindle. Mol Biol Cell. 2007 Oct;18(10):3800-9.
Download PDF Supplemental Data Fields S. Molecular biology. Site-seeing by sequencing. Science. 2007 Jun 8;316(5830):1441-2. Download PDF Gerstein M, Seringhaus M, Fields S. Structured digital abstract makes text mining easy. Nature. 2007 May 10;447(7141):142. Download PDF Zhang Z, Hesselberth JR, Fields S. Genome-wide identification of spliced introns using a tiling microarray. Genome Res. 2007 Mar 9. Download PDF Supplemental Data |
2005 |
Kaeberlein M, Hu D, Kerr EO, Tsuchiya M, Westman EA, Dang N, Fields S, Kennedy BK. Increased Life Span due to Calorie Restriction in Respiratory-Deficient Yeast. PLoS Genet. 2005 Nov 25;1(5):e69.
Download PDF Kaeberlein M, Powers RW 3rd, Steffen KK, Westman EA, Hu D, Dang N, Kerr EO, Kirkland KT, Fields S, Kennedy BK. Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients. Science. 2005 Nov 18;310(5751):1193-6. Download PDF Lacount DJ, Vignali M, Chettier R, Phansalkar A, Bell R, Hesselberth JR, Schoenfeld LW, Ota I, Sahasrabudhe S, Kurschner C, Fields S, Hughes RE. A protein interaction network of the malaria parasite Plasmodium falciparum. Nature. 2005 Nov 3;438(7064):103-7. Download PDF Fields S. High-throughput two-hybrid analysis. The promise and the peril. FEBS Journal. 2005;272(21):5391-9. Download PDF Miller JP, Lo RS, Ben-Hur A, Desmarais C, Stagljar I, Noble WS, et al. Large-scale identification of yeast integral membrane protein interactions. Proceedings of the National Academy of Sciences of the United States of America. 2005;102(34):12123-8. Download PDF Supplemental Data Natter K, Leitner P, Faschinger A, Wolinski H, McCraith S, Fields S, et al. The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy. Mol Cell Proteomics. 2005;4(5):662-72. Download PDF Devit M, Cullen PJ, Branson M, Sprague GF, Jr., Fields S. Forcing interactions as a genetic screen to identify proteins that exert a defined activity. Genome Res. 2005;15(4):560-5. Download PDF Fields S, Johnston M. Cell biology. Whither model organism research? Science. 2005;307(5717):1885-6. Download PDF Kaeberlein M, Kirkland KT, Fields S, Kennedy BK. Genes determining yeast replicative life span in a long-lived genetic background. Mech Ageing Dev. 2005;126(4):491-504. Download PDF Kaeberlein M, McDonagh T, Heltweg B, Hixon J, Westman EA, Caldwell SD, et al. Substrate specific activation fo sirtuins by resveratrol.. J Biol Chem. 2005;280(17):17038-45. Download PDF Jeffress M, Fields S. Identification of putative Plasmodium falciparum mefloquine resistance genes. Mol Biochem Parasitol. 2005;139(2):133-9. Download PDF |
2004 |
Antoniacci LM, Kenna MA, Uetz P, Fields S, Skibbens RV. The spindle pole body assembly component mps3p/nep98p functions in sister chromatid cohesion. J Biol Chem. 2004;279(47):49542-50.
Download PDF Kaeberlein M, Kirkland KT, Fields S, Kennedy BK. Sir2-independent life span extension by calorie restriction in yeast. PLoS Biol. 2004;2(9):E296. Download PDF Alexandrov A, Vignali M, LaCount DJ, Quartley E, de Vries C, De Rosa D, et al. A facile method for high-throughput co-expression of protein pairs. Mol Cell Proteomics. 2004;3(9):934-8. Download PDF Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH. A tethered catalysis two-hybrid system to identify protein-protein interactions requiring post-translational modifications. Nat Biotechnol. 2004;22(7):888-92. Download PDF Tucker CL, Fields S. Quantitative genome-wide analysis of yeast deletion strain sensitivities to oxidative and chemical stress. Comp Funct Genomics. 2004;5(3):216-24. Download PDF Supplemental Data |
2003 |
Hazbun TR, Malmstrom L, Anderson S, Graczyk BJ, Fox B, Riffle M, et al. Assigning function to yeast proteins by integration of technologies. Mol Cell. 2003;12(6):1353-65.
Download PDF Tucker CL, Fields S. Lethal combinations. Nat Genet. 35. United States2003. p. 204-5. Download PDF Guo M, Aston C, Burchett SA, Dyke C, Fields S, Rajarao SJ, et al. The yeast G protein alpha subunit Gpa1 transmits a signal through a novel effector RNA-binding protein Scp160. Mol Cell. 2003;12(2):517-24. Download PDF Shang C, Hazbun TR, Cheeseman IM, Aranda J, Fields S, Drubin DG, et al. Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p. Mol Biol Cell. 2003;14(8):3342-55. Download PDF Kitagawa K, Abdulle R, Bansal PK, Cagney G, Fields S, Hieter P. Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Mol Cell. 2003;11(5):1201-13. Download PDF Phizicky E, Bastiaens PI, Zhu H, Snyder M, Fields S. Protein analysis on a proteomic scale. Nature. 2003;422(6928):208-15. Download PDF Pot I, Measday V, Snydsman B, Cagney G, Fields S, Davis TN, et al. Chl4p and Iml3p are two new members of the budding yeast outer kinetochore. Mol Biol Cell. 2003;14(2):460-76. Download PDF |
2002 |
Stagljar I, Fields S. Analysis of membrane protein interactions using yeast-based technologies.. Trends Biochem Sci. 2002;27(11):559-63.
Download PDF Fields S. Forward. Discovering genomics, proteomics, and bioinformatics by Campbell AM, Heyer LJ. First edition ed: San Francisco: Benjamin Cummings; 2002. p. ix. Hazbun TR, Fields S. A genome-wide screen for site-specific DNA binding proteins. Mol Cell Proteomics. 2002;1(7):538-43. Download PDF Phizicky EM, Martzen MR, McCraith SM, Spinelli SL, Xing F, Shull NP, et al. Biochemical genomics approach to map activities to genes. Methods Enzymol. 2002;350:546-59. von Mering C, Krause R, Snel B, Cornell M, Oliver SG, Fields S, et al. Comparative assessment of large-scale data sets of protein-protein interactions. Nature. 2002;417(6887):399-403. Download PDF Fields S, Johnston M. Genomics. A crisis in postgenomic nomenclature. Science. 2002;296(5568):671-2. Download PDF Burchett SA, Flanary P, Aston C, Jiang L, Young KH, Uetz P, et al. Regulation of stress response signaling by the N-terminal DEP (dishevelled/EGL-10/pleckstrin) domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae. J Biol Chem. 2002;277(25):22156-67. Download PDF Measday V, Hailey DW, Pot I, Givan SA, Hyland KM, Cagney G, et al. Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev. 2002;16(1):101-13. Download PDF Muchowski PJ, Ning K, D'Souza-Schorey C, Fields S. Requirement of an intact microtubule cytoskeleton for the aggregation of an amino-terminal fragment of huntingtin. Proc Natl Acad Sci U S A. 2002;99(2):727-32. Download PDF Gera JF, Hazbun TR, Fields S. Array-based methods for identifying protein-protein and protein-nucleic acid interactions. Methods Enzymol. 2002;350:499-512. Tong AH, Drees B, Nardelli G, Bader GD, Brannetti B, Castagnoli L, et al. A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules. Science. 2002;295(5553):321-4. Download PDF |
2001 |
Hughes RE, Lo RS, Davis C, Strand AD, Neal CL, Olson JM, et al. Altered transcription in yeast expressing polyglutamine. Proc Natl Acad Sci U S A. 2001;98(23):13201-6.
Download PDF Cagney G, Uetz P, Fields S. Two-hybrid analysis of the Saccharomyces cerevisiae 26S proteasome. Physiol Genomics. 2001;7(1):27-34. Download PDF Tucker CL, Fields S. A yeast sensor of ligand binding. Nat Biotechnol. 2001;19(11):1042-6. Download PDF Fields S. The interplay of biology and technology. Proc Natl Acad Sci U S A. 2001;98(18):10051-4. Download PDF Drees BL, Sundin B, Brazeau E, Caviston JP, Chen GC, Guo W, et al. A protein interaction map for cell polarity development. J Cell Biol. 2001;154(3):549-71. Download PDF Fields S. Proteomics. Proteomics in genomeland. Science. 2001;291(5507):1221-4. Download PDF Wood J, Frederickson RM, Fields S, Patel AH. Hepatitis C virus 3' X region interacts with human ribosomal proteins. J Virol. 2001;75(3):1348-58. Download PDF Johnston M, Fields S. Proteomics and genomics Presidential preference, protein purpose. Current Opinion in Chemical Biology. 2001 Feb 1;5(1):13-14. Available from, DOI: 10.1016/S1367-5931(00)00174-5. Fields, S. Two hybrid proteins to many hybrid proteins. ASM News 67:135-143. 2001. Hazbun TR, Fields S. Networking proteins in yeast.. Proc Natl Acad Sci U S A. 2001;98(8):4277-8. Download PDF |
1999 |
Drees BL. Progress and variations in two-hybrid and three-hybrid technologies. Curr Opin Chem Biol. 1999;3(1):64-70.
Download PDF Martzen MR, McCraith SM, Spinelli SL, Torres FM, Fields S, Grayhack EJ, et al. A biochemical genomics approach for identifying genes by the activity of their products. Science. 1999;286(5442):1153-5. Download PDF Norman TC, Smith DL, Sorger PK, Drees BL, O'Rourke SM, Hughes TR, et al. Genetic selection of peptide inhibitors of biological pathways. Science. 1999;285(5427):591-5. Download PDF Fields S, Kohara Y, Lockhart DJ. Functional genomics. Proc Natl Acad Sci U S A. 1999;96(16):8825-6. Download PDF Fields, S. Two-hybrid and related systems. In Encyclopedia of Life Sciences. 1999. London: Macmillan. Zhang B, Kraemer B, SenGupta D, Fields S, Wickens M. Yeast three-hybrid system to detect and analyze interactions between RNA and protein. Methods Enzymol. 1999;306:93-113. Wright ME, Han DK, Carter L, Fields S, Schwartz SM, Hockenbery DM. Caspase-3 inhibits growth in Saccharomyces cerevisiae without causing cell death. FEBS Lett. 1999;446(1):9-14. Download PDF Hughes RE, Fields S. The PDZ domain as you like it. Nat Biotechnol. 1999;17(2):132-3. Sengupta DJ, Wickens M, Fields S. Identification of RNAs that bind to a specific protein using the yeast three-hybrid system. Rna. 1999;5(4):596-601. Download PDF |